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LB medium bottle and LB agar plate Plate medium agar LB. Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani, intended LB to stand for lysogeny broth, [1] but LB has also come to colloquially mean Luria broth, Lennox broth, life broth or Luria–Bertani medium. [2]
Prepare a bacterial culture in LB broth; Before starting the main procedure, use the required volume of the previously made culture to inoculate the required volume of fresh LB broth; Pellet the cells by centrifuging at 4°C at 4000 rpm for 10 minutes
Nutrient broth has the same composition, but lacks agar. [ 2 ] [ 3 ] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.
Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. This nutrient-rich microbial broth contains peptides , amino acids , water soluble vitamins and glucose in a low-salt formulation.
When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered.
This method is a faster process than dry heat sterilization. Steam sterilization is performed using an autoclave, sometimes called a converter or steam sterilizer. The object or liquid is placed in the autoclave chamber, which is then sealed and heated using pressurized steam to a temperature set point for a defined period of time.
Place the bowl of broth into the ice bath and chill, stirring occasionally, until broth has cooled to 40°F, about one hour. Once chilled, the broth can be placed in the refrigerator or freezer.
Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms. [2] It provides a source of amino acids for the growing bacteria .