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In cell respiration, the proton pump uses energy to transport protons from the matrix of the mitochondrion to the inter-membrane space. [1] It is an active pump that generates a proton concentration gradient across the inner mitochondrial membrane, because there are more protons outside the matrix than inside.
An ion gradient has potential energy and can be used to power chemical reactions when the ions pass through a channel (red). Hydrogen ions, or protons, will diffuse from a region of high proton concentration to a region of lower proton concentration, and an electrochemical concentration gradient of protons across a membrane can be harnessed to ...
The proton gradient can be generated through either noncyclic or cyclic photophosphorylation. Of the proteins that participate in noncyclic photophosphorylation, photosystem II (PSII), plastiquinone, and cytochrome b 6 f complex directly contribute to generating the proton gradient. For each four photons absorbed by PSII, eight protons are ...
The complex contains coordinated copper ions and several heme groups. At the same time, eight protons are removed from the mitochondrial matrix (although only four are translocated across the membrane), contributing to the proton gradient. The exact details of proton pumping in Complex IV are still under study. [9] Cyanide is an inhibitor of ...
The microelectrode method for measuring pH i consists of placing a very small electrode into the cell’s cytosol by making a very small hole in the plasma membrane of the cell. [19] Since the microelectrode has fluid with a high H+ concentration inside, relative to the outside of the electrode, there is a potential created due to the pH ...
Permitting one ion or molecule to move down an electrochemical gradient, but possibly against the concentration gradient where it is more concentrated to that where it is less concentrated, increases entropy and can serve as a source of energy for metabolism (e.g. in ATP synthase). The energy derived from the pumping of protons across a cell ...
H+-ATPase energizes nutrient uptake by establishing an electrochemical proton gradient that will drive secondary active transport. H+-ATPase uses the energy of ATP to pump H+ protons out of the cytoplasm of the cell. [13] This creates and maintains an electrochemical gradient of H+ from outside the cell to inside the cell.
The hydrogen ions (protons) generated by the oxidation of water help to create a proton gradient that is used by ATP synthase to generate ATP. The energized electrons transferred to plastoquinone are ultimately used to reduce NADP + to NADPH or are used in non-cyclic electron flow. [1]