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The slice preparation or brain slice is a laboratory technique in electrophysiology that allows the study of neurons from various brain regions in isolation from the rest of the brain, in an ex-vivo condition. Brain tissue is initially sliced via a tissue slicer then immersed in artificial cerebrospinal fluid (aCSF) for stimulation and/or ...
Organotypic brain slices are an in vitro model that replicates in vivo physiology with additional throughput and optical benefits, [8] thus pairing well with microfluidic devices. Brain slices have advantages over primary cell culture in that tissue architecture is preserved and multicellular interactions can still occur.
Each of the resulting 1,871 "slices" was photographed in both film and digital, yielding 15 gigabytes of data. In 2000, the photos were rescanned at a higher resolution, yielding more than 65 gigabytes. The female cadaver was cut into slices at 0.33-millimeter intervals, resulting in some 40 gigabytes of data.
Nevertheless, even with the highest standards of tissue handling, slice preparation induces rapid and robust phenotype changes of the brain's major immune cells, microglia, which must be taken into consideration when using this model. [6]
Long-term potentiation (LTP) is a persistent increase in synaptic strength following high-frequency stimulation of a chemical synapse.Studies of LTP are often carried out in slices of the hippocampus, an important organ for learning and memory.
It is measured by the size of voxels, as in MRI. A voxel is a three-dimensional rectangular cuboid, whose dimensions are set by the slice thickness, the area of a slice, and the grid imposed on the slice by the scanning process. Full-brain studies use larger voxels, while those that focus on specific regions of interest typically use smaller sizes.
Stained brain slice images which include the "corpus callosum" at the BrainMaps project; Comparative Neuroscience at Wikiversity; NIF Search – Corpus callosum Archived 2016-03-04 at the Wayback Machine via the Neuroscience Information Framework; National Organization for Disorders of the Corpus Callosum; A 3D model of corpus callosum
A 2-D model of cortical sensory homunculus. A cortical homunculus (from Latin homunculus 'little man, miniature human' [1] [2]) is a distorted representation of the human body, based on a neurological "map" of the areas and portions of the human brain dedicated to processing motor functions, and/ or sensory functions, for different parts of the body.