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The isoelectric point (pI, pH(I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean. The standard nomenclature to represent the isoelectric point is pH(I). [1] However, pI is also used. [2] For brevity, this article uses pI.
In chemistry, a zwitterion (/ ˈ t s v ɪ t ə ˌ r aɪ ə n / TSVIT-ə-ry-ən; from German Zwitter 'hermaphrodite'), also called an inner salt or dipolar ion, [1] is a molecule that contains an equal number of positively and negatively charged functional groups. [2] 1,2-dipolar compounds, such as ylides, are sometimes excluded from the ...
In approximately neutral aqueous solution (pH ≅ 7), the basic amino group is mostly protonated and the carboxylic acid is mostly deprotonated, so that the predominant species is the zwitterion H 3 N + −RCH−COO −. The pH at which the average charge is zero is known as the molecule's isoelectric point.
[72] [73] [74] The isoelectric point of a given molecule is a function of its pK values, so different molecules have different isoelectric points. This permits a technique called isoelectric focusing, [75] which is used for separation of proteins by 2-D gel polyacrylamide gel electrophoresis. Buffer solutions also play a key role in analytical ...
Zeta potential titration is a titration of heterogeneous systems, for example colloids and emulsions.Solids in such systems have very high surface area.This type of titration is used to study the zeta potential of these surfaces under different conditions.
The following other wikis use this file: Usage on af.wikipedia.org Zwitterioon; Usage on azb.wikipedia.org سولفامیک اسید; Usage on bs.wikipedia.org
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The following table presents pK a values at 20 °C. Values change by about 0.01 per degree of temperature. [1] [3] Good's original 1966 paper had two older buffers (marked with italics) for comparison.