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Micro-mass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. A commonly applied definition of tissue engineering, as stated by Langer [3] and Vacanti, [4] is "an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve [Biological tissue] function or a ...
Partial-thickness tissue engineering uses one type of cell layer, this can be in monolayers or multilayers. Monolayer epithelial sheets suffice for the study of the basic biology of oral mucosa, for example its responses to stimuli such as mechanical stress, growth factor addition and radiation damage .
The first study on tissue engineering of heart valves was published in 1995. [11] During 1995 and 1996, Shinoka used a scaffold made of polyglycolic acid (PGA), approved by the FDA for human implantation, and seeded it with sheep endothelial cells and fibroblasts with the goal of replacing a sheep's pulmonary valve leaflet. [ 22 ]
Fixed prosthodontics is the branch of prosthodontics that focuses on dental prostheses that are permanently affixed (fixed). Crowns , bridges (fixed dentures), inlays , onlays , and veneers are some examples of indirect dental restorations .
A removable partial denture (RPD) is a denture for a partially edentulous patient who desires to have replacement teeth for functional or aesthetic reasons and who cannot have a bridge (a fixed partial denture) for any reason, such as a lack of required teeth to serve as support for a bridge (i.e. distal abutments) or financial limitations.
There has been a decline in both the prevalence and incidence of tooth loss within the last decades; [1] [2] people retain their natural dentition for longer. Nonetheless there is still a great demand for complete dentures as more than 10% of adults aged 50–64 are completely edentulous, with age, smoking status and socioeconomic status being significant risk factors. [2]
Muscle tissue engineering is a subset of the general field of tissue engineering, which studies the combined use of cells and scaffolds to design therapeutic tissue implants. Within the clinical setting, muscle tissue engineering involves the culturing of cells from the patient's own body or from a donor, development of muscle tissue with or ...
The sample of tissue is immersed in fixative solution for a set period of time. The fixative solution must have a volume at least 10 times greater than the volume of the tissue. [6] In order for fixation to be successful, the fixative must diffuse throughout the entire tissue, so tissue size and density, as well as type of fixative must be ...