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These explain how T7 polymerase binds to DNA and transcribes it. The N-terminal domain moves around as the elongation complex forms. The ssRNAP holds a DNA-RNA hybrid of 8bp. [3] A beta-hairpin specificity loop (residues 739-770 in T7) recognizes the promoter; swapping it out for one found in T3 RNAP makes the polymerase recognize T3 promoters ...
The T7 gene is itself under the control of a lac promoter. Normally, both the lac promoter and the T7 promoter are repressed in the E. coli cell by the Lac repressor. In order to initiate transcription, an inducer must bind to the lac repressor and prevent it from inhibiting the gene expression of the T7 gene. Once this happens, the gene can be ...
T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin, [1] in order to carry out its function
The decision to file this lawsuit to rightfully protect himself and his family was made after Ms. Lively not only filed against him first in Texas but, indicated she intended to name him in yet ...
The season of the mint-flavored, green-tinted shake is nearly upon us. McDonald's Shamrock Shake, a milkshake made with vanilla soft-serve ice cream, artificially flavored minty green syrup and ...
In December 2012, Allwinner announced the availability of two ARM Cortex-A7 MPCore powered products, the dual-core Allwinner A20 and quad-core Allwinner A31. Production of the A31 started in September 2012 and end products, mostly high-end tablets from Chinese manufacturers, appeared on the market in early 2013, including the Onda V972.
"The microchip is not a GPS device and cannot track your animal if it gets lost," according to the AVMA. How to update a microchip. Pet owners can check with their veterinarians, many of whom have ...
In a 1945 study by Demerec and Fano, [4] T7 was used to describe one of the seven phage types (T1 to T7) that grow lytically on Escherichia coli. [5] Although all seven phages were numbered arbitrarily, phages with odd numbers, or T-odd phages, were later discovered to share morphological and biochemical features that distinguish them from T-even phages. [6]