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Microorganisms growing on an agar plate. Sterilization (British English: sterilisation) refers to any process that removes, kills, or deactivates all forms of life (particularly microorganisms such as fungi, bacteria, spores, and unicellular eukaryotic organisms) and other biological agents (such as prions or viruses) present in fluid or on a specific surface or object. [1]
It is sold in dissolvable tablets which are then mixed with cold water and placed in a lidded bucket. This method of bottle sterilization is marketed as "The Milton Method". A 1:20 solution is isotonic with body fluids. 1:4 dilution is used for wound management applications; this contains 0.25% (w/v) available chlorine and has a pH of 10.5–11.2.
There are physical, chemical, and biological indicators that can be used to ensure that an autoclave reaches the correct temperature for the correct amount of time. If a non-treated or improperly treated item can be confused for a treated item, then there is the risk that they will become mixed up, which, in some areas such as surgery, is critical.
Chemical sterilization is generally more expensive than steam sterilization and therefore it is used for instruments that cannot be disinfected otherwise. After the instruments have been soaked in the chemical solutions, they must be rinsed with sterile water which will remove the residues from the disinfectants.
used for steam sterilization A pressure cooker: used as a portable autoclave Biological and chemical indicators: Used to ascertain if a certain process has been completed, e.g. spores used in an autoclave are killed if autoclaving is properly done Filters: •Candle filter:
A portable, battery-powered, low-pressure mercury-vapour discharge lamp for water sterilization Cutaway model of UV disinfection unit used in water treatment plants. Ultraviolet disinfection of water is a purely physical, chemical-free process.
Pascalization, bridgmanization, high pressure processing (HPP) [1] or high hydrostatic pressure (HHP) processing [2] is a method of preserving and sterilizing food, in which a product is processed under very high pressure, leading to the inactivation of certain microorganisms and enzymes in the food. [3]
Pressure sterilization is the prevailing method used for medical sterilization of heat-resistant tools, and for sterilization of materials for microbiology and other fields calling for aseptic technique. [citation needed] In cases when items need to be sterilized for immediate use, flash sterilization may be employed. [3]