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The van Deemter equation is a hyperbolic function that predicts that there is an optimum velocity at which there will be the minimum variance per unit column length and, thence, a maximum efficiency. The van Deemter equation was the result of the first application of rate theory to the chromatography elution process.
The partition coefficient, abbreviated P, is defined as a particular ratio of the concentrations of a solute between the two solvents (a biphase of liquid phases), specifically for un- ionized solutes, and the logarithm of the ratio is thus log P. [10]: 275ff When one of the solvents is water and the other is a non-polar solvent, then the log P ...
Gel permeation chromatography. Gel permeation chromatography (GPC) [1] is a type of size-exclusion chromatography (SEC), that separates high molecular weight or colloidal analytes on the basis of size or diameter, typically in organic solvents. The technique is often used for the analysis of polymers. As a technique, SEC was first developed in ...
Chromatography – a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. Eluent (sometimes spelled eluant) – the solvent or solvent fixure used in elution chromatography and is synonymous with mobile phase.
Cytiva, Bio-Rad, Bio-Works, emp Biotech, Knauer, Phenomenex. Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins ...
Resolution (chromatography) Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1][2][3][4]
In gas chromatography, the Kovats retention index (shorter Kovats index, retention index; plural retention indices) is used to convert retention times into system-independent constants. The index is named after the Hungarian-born Swiss chemist Ervin Kováts, who outlined the concept in the 1950s while performing research into the composition of ...
The fundamental resolution equation is used in chromatography to help relate adjustable chromatographic parameters to resolution, and is as follows: R s = [N 1/2 /4] [ (α-1)/α] [k 2 '/ (1+k 2 ')], where. N = Number of theoretical plates. α = Selectivity Term = k 2 '/k 1 '. The [N 1/2 /4] term is the column factor, the [ (α-1)/α] term is ...