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The minimum bactericidal concentration (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. [1] It can be determined from broth dilution minimum inhibitory concentration (MIC) tests by subculturing to agar plates that do not contain the test agent.
While consuming a low dose of pathogenic bacterium is associated with a low probability of disease, infection is still possible. This contributes to sporadic cases of food-borne illness in the population. There is no bacterial concentration in food below which a lack of epidemic is guaranteed.
Bacteria are marked as sensitive, resistant, or having intermediate resistance to an antibiotic based on the minimum inhibitory concentration (MIC), which is the lowest concentration of the antibiotic that stops the growth of bacteria. The MIC is compared to standard threshold values (called "breakpoints") for a given bacterium and antibiotic. [28]
6 month mortality is >=60% with fluconazole-based therapy and 40% with amphotericin-based therapy in research studies in low and middle income countries. [27] Anthrax, gastrointestinal: Bacterial Unvaccinated and untreated > 50% [7]: 27 Tetanus, Generalized Bacterial Unvaccinated and untreated 50% CFR drops to [10–20]% with effective treatment.
While the MIC is the lowest concentration of an antibacterial or antifungal agent necessary to inhibit visible growth, the minimum bactericidal concentration (MBC) is the minimum concentration of an antibacterial agent that results in bacterial death. It is defined by the inability to re-culture bacteria, and the closer the MIC is to the MBC ...
The standard can be compared visually to a suspension of bacteria in sterile saline or nutrient broth. If the bacterial suspension is too turbid, it can be diluted with more diluent. If the suspension is not turbid enough, more bacteria can be added. McFarland nephelometer standards:{2}
The purpose of plate counting is to estimate the number of cells present based on their ability to give rise to colonies under specific conditions of temperature, time, and nutrient medium. Theoretically, one viable cell can give rise to a colony through replication. However, solitary cells are the exception in nature, and in most cases the ...
Ishigaki and Sleigh (2001) found that C. roenbergensis ceased to reproduce when the concentration of bacteria that they were grazing on became less than 2.0×10 7 cells ml −1. [8] Other flagellates were able to multiply at much lower bacterial concentrations, indicating that bacterial concentration is a limiting factor for Cafeteria.