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where Δν̃ is the Raman shift expressed in wavenumber, λ 0 is the excitation wavelength, and λ 1 is the Raman spectrum wavelength. Most commonly, the unit chosen for expressing wavenumber in Raman spectra is inverse centimeters (cm −1). Since wavelength is often expressed in units of nanometers (nm), the formula above can scale for this ...
The spectrum of intensity differences recorded over a range of wavenumbers reveals information about chiral centres in the sample molecule. Raman optical activity can be observed in a number of forms, depending on the polarization of the incident and the scattered light.
The inverse Raman effect is a form of Raman scattering first noted by W. J. Jones and Boris P. Stoicheff. In some circumstances, Stokes scattering can exceed anti-Stokes scattering; in these cases the continuum (on leaving the material) is observed to have an absorption line (a dip in intensity) at ν L +ν M.
Stimulated Raman spectroscopy, also referred to as stimulated Raman scattering (SRS), is a form of spectroscopy employed in physics, chemistry, biology, and other fields. . The basic mechanism resembles that of spontaneous Raman spectroscopy: a pump photon, of the angular frequency , which is scattered by a molecule has some small probability of inducing some vibrational (or rotational ...
Raman amplification / ˈ r ɑː m ən / [1] is based on the stimulated Raman scattering (SRS) phenomenon, when a lower frequency 'signal' photon induces the inelastic scattering of a higher-frequency 'pump' photon in an optical medium in the nonlinear regime. As a result of this, another 'signal' photon is produced, with the surplus energy ...
A difference in selection rules is visible: Some bands appear only in the bulk-phase Raman spectrum or only in the SERS spectrum. Surface-enhanced Raman spectroscopy or surface-enhanced Raman scattering ( SERS ) is a surface-sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces or by nanostructures ...
By using Raman microspectroscopy, in vivo time- and space-resolved Raman spectra of microscopic regions of samples can be measured. Sampling is non-destructive and water, media, and buffers typically do not interfere with the analysis. Consequently, in vivo time- and space-resolved Raman spectroscopy is suitable to examine proteins, cells and ...