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REBASE contains an extensive set of references, sites of recognition and cleavage, sequences and structures. It also contains information on the commercial availability of each enzyme. REBASE is one of the longest running biological databases having its roots in a collection of restriction enzymes maintained by Richard J. Roberts since before ...
In molecular biology, the Cfr10I/Bse634I family of restriction endonucleases includes the type II restriction endonucleases Cfr10I and Bse634I. They exhibit a conserved tetrameric architecture that is of functional importance, wherein two dimers are arranged, back-to-back, with their putative DNA-binding clefts facing opposite directions.
New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. [2] It also provides products and services supporting genome editing , synthetic biology and next-generation sequencing . [ 3 ]
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A rare-cutter enzyme is a restriction enzyme with a recognition sequence which occurs only rarely in a genome. An example is NotI, which cuts after the first GC of a 5'-GCGGCCGC-3' sequence; restriction enzymes with seven and eight base pair recognition sequences are often also called rare-cutter enzymes (six bp recognition sequences are much more common).
This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site. The following information is given for each enzyme: Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, [1] [2] and bibliographical references. Note: When ...
A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria.One special kind of restriction enzymes is the class of "homing endonucleases", these being present in all three domains of life, although their function seems to be very different from one domain to another.
It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a high CpG content. Due to the high cost and depth of sequencing to analyze methylation status in the entire genome, Meissner et al. developed this technique in 2005 to reduce the amount of nucleotides required to sequence to 1% of the genome. [1]