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One of the most significant contributions of the experiment was its role in establishing the concept of the gut-brain axis. It was one of the first studies to provide evidence for what would later be recognized as a complex communication system between the digestive system and the central nervous system in regulating hunger. [13]
A common laboratory-scale mechanical method for cell disruption uses glass, ceramic, or steel beads, 0.1–2 mm (0.004–0.08 in) in diameter, mixed with a sample suspended in an aqueous solution. First developed by Tim Hopkins in the late 1970s, the sample and bead mix is subjected to high level agitation by stirring or shaking.
This experiment works because it shows how salivary amylase – a type of enzyme that exists in our saliva – breaks down the starch in the bread into a sweet-tasting sugar.
Micrococcal nuclease (MNase) was first discovered in S. aureus in 1956, [10] protein crystallized in 1966, [11] and characterized in 1967. [12] MNase digestion of chromatin was key to early studies of chromatin structure; being used to determine that each nucleosomal unit of chromatin was composed of approximately 200bp of DNA. [13]
However, experiments about the duration of the digestion process showed that after 3 h there is enough material for successful mass spectrometric analysis. [38] Furthermore, the optimisation of the conditions for the protease in temperature and pH allows for the completion of the digestion of a sample in 30 min. [16]
Lab notebook with the complete record of the experiments underlying a published paper. [1] Chemistry stencils that used to be used for drawing equipment in lab notebooks. A laboratory notebook ( colloq. lab notebook or lab book ) is a primary record of research .
In analytical chemistry, sample preparation (working-up) refers to the ways in which a sample is treated prior to its analyses. Preparation is a very important step in most analytical techniques, because the techniques are often not responsive to the analyte in its in-situ form, or the results are distorted by interfering species .
A cannula in a cow's side. A cannulated cow or fistulated cow refers to a cow that has been surgically fitted with a cannula. [1] A cannula acts as a porthole-like device that allows access to the rumen of a cow, to perform research and analysis of the digestive system and to allow veterinarians to transplant rumen contents from one cow to another.