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To perform the OF-glucose test, two tubes of OF-glucose medium are inoculated with the test organism. A layer of mineral oil is added to the top of the deep in one of the tubes to create anaerobic conditions. Oil is not added to the other tube to allow for aerobic conditions. The tubes are then incubated for 24–48 hours.
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
Substance in water + 3 mL Benedict's solution, then boil for few minutes and allow to cool. Red, green, or yellow precipitate is obtained Reducing sugar, such as glucose, is present Substance in water + 3 mL Benedict's solution, then boil for few minutes and allow to cool. Solution remains clear or is a little blue Reducing sugar is not present
The aqueous solution in the classical reaction contains glucose, sodium hydroxide and methylene blue. [14] In the first step an acyloin of glucose is formed. The next step is a redox reaction of the acyloin with methylene blue in which the glucose is oxidized to diketone in alkaline solution [6] and methylene blue is reduced to colorless leucomethylene blue.
The Gerber method is the primary testing method in Europe and much of the world. [2] The fairly similar Babcock test is used primarily in the United States, although the Gerber method also enjoys significant use in the U.S. as well. [3] The Gerber method was developed and patented by Dr. Niklaus Gerber of Switzerland in 1891. [4]
The volumetric titration is based on the same principles as the coulometric titration, except that the anode solution above now is used as the titrant solution. The titrant consists of an alcohol (ROH), base (B), SO 2 and a known concentration of I 2. Pyridine has been used as the base in this case. One mole of I 2 is consumed for each mole of ...
The test employs the Trinder reagent, and is a colour change test resulting from the Trinder reaction. The Trinder reagent, named after P. Trinder of the Biochemistry Department of the Royal Infirmary in Sunderland (see the article listed in further reading ), comprises an aminoantipyrine (such as 4-aminoantipyrine ) and phenol (p-hydroxybenzene).
Hyperglycemic clamp technique: The plasma glucose concentration is acutely raised to 125 mg/dl above basal levels by a continuous infusion of glucose. This hyperglycemic plateau is maintained by adjustment of a variable glucose infusion, based on the rate of insulin secretion and glucose metabolism. Because the plasma glucose concentration is ...