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NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence. [2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR ...
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
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Paint thinners are diluents — solvents used to adjust the viscosity of paint, as well as similar preparations such as varnish and lacquer, by dilution. [1] [2] Organic solvents labelled "paint thinner" are usually turpentine or white spirits / mineral spirits for the use of diluting oil-based paints only.
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The indoor use of solvent-based primer results in increased solvent exposure due to build-up of fumes in the air. One way to reduce the amount of solvent in the air is to use fans to renew the air. The Air Change Index (ACI) of a solvent is an indication of the margin of safety of a solvent. The lower the ACI, the less air turnover needed. [2] [3]
The second active ingredient is an organic solvent such as 2-butoxyethanol (ethylene glycol monobutyl ether, trade name butyl cellosolve) that acts as a wetting agent and provides a protective primer layer in conjunction with an organic polymer emulsion.
Primer Premier is a bioinformatics software used for various PCR applications. It supports the design of degenerate primers for amplifying a related set of nucleotide sequences for the detection of common traits amongst organisms, as well as to determine heredity. [1] The software also designs tagged and nested primers for multiplex PCR ...