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Methods of cell sorting fall into two major categories: fluorescence activated cell sorting (FACS) and immunomagnetic cell sorting. [2] Due to many years of refinement and increased demand for cell separation however, researchers are working to develop microfluidic sorting devices that have many benefits in comparison to the main types of fluorescence-activated cell sorting and immunomagnetic ...
CyTOF. Cytometry by time of flight, or CyTOF, is an application of mass cytometry used to quantify labeled targets on the surface and interior of single cells. CyTOF allows the quantification of multiple cellular components simultaneously using an ICP-MS detector. CyTOF takes advantage of immunolabeling to quantify proteins, carbohydrates or ...
A magnetic-labelled receptor that binds to annexin is added to sperm. Inside normal cells, phosphatidylserine molecules are located within the cell membrane towards the cytoplasm. Nevertheless, in those cells that initiate the apoptotic process phosphatidylserine instead faces the cell membrane outer side, binding to the annexin conjugate.
Marching squares. In computer graphics, marching squares is an algorithm that generates contours for a two-dimensional scalar field (rectangular array of individual numerical values). A similar method can be used to contour 2D triangle meshes. The contours can be of two kinds:
Genome (DNA) sequencing. Single-cell DNA genome sequencing involves isolating a single cell, amplifying the whole genome or region of interest, constructing sequencing libraries, and then applying next-generation DNA sequencing (for example Illumina, Ion Torrent). Single-cell DNA sequencing has been widely applied in mammalian systems to study ...
Protein targeting or protein sorting is the biological mechanism by which proteins are transported to their appropriate destinations within or outside the cell. [ 1 ] [ 2 ] [ note 1 ] Proteins can be targeted to the inner space of an organelle , different intracellular membranes , the plasma membrane , or to the exterior of the cell via secretion .
Timsort is a hybrid, stable sorting algorithm, derived from merge sort and insertion sort, designed to perform well on many kinds of real-world data. It was implemented by Tim Peters in 2002 for use in the Python programming language. The algorithm finds subsequences of the data that are already ordered (runs) and uses them to sort the ...
The functioning of the individual cells in multicellular organisms can only be completely explained in the context of identifying their exact location in the body. [4] Spatial transcriptomics techniques sought to elucidate cells’ properties this way. Below, we look into the methods that connect gene expression to the spatial organization of ...