Ads
related to: dead time hplc
Search results
Results From The WOW.Com Content Network
The total dead time of a detection system is usually due to the contributions of the intrinsic dead time of the detector (for example the ion drift time in a gaseous ionization detector), of the analog front end (for example the shaping time of a spectroscopy amplifier) and of the data acquisition (the conversion time of the analog-to-digital converters and the readout and storage times).
The time it takes for the solution to travel from the mixing point to the observation point is referred to as the "dead time." The minimum injection volume depends on the size of the mixing cell. Once enough solution has been injected to completely replace the previous one, the system reaches a stationary state, and the flow is stopped.
HPLC has many applications in both laboratory and clinical science. It is a common technique used in pharmaceutical development, as it is a dependable way to obtain and ensure product purity. [59] While HPLC can produce extremely high quality (pure) products, it is not always the primary method used in the production of bulk drug materials. [60]
Technical and historical variations of this effect have been referred to as time slicing, view morphing, temps mort (French: "dead time") and virtual cinematography. The term "bullet time" was first used with reference to the 1999 film The Matrix, [2] and later in reference to the slow motion effects in the 2001 video game Max Payne.
In reverse phase HPLC, the solute with the greatest polarity will interact less with the stationary phase and spend more time in the mobile phase. As the polarity of the components decreases, the time spent in the column increases. Thus, a separation of components is achieved based on polarity. [4]
The velocity of the charged particle after acceleration will not change since it moves in a field-free time-of-flight tube. The velocity of the particle can be determined in a time-of-flight tube since the length of the path (d) of the flight of the ion is known and the time of the flight of the ion (t) can be measured using a transient digitizer or time to digital converter.
This mismatch will therefore decrease the interaction with the column and will result in a reduced retention time compared to the homoduplexes in the chromatographic separation process. To observe the phenomenon of separation, the DHPLC method uses a column of a non-grafted porous stationary phase composed of polystyrene - divinylbenzene alkyl.
The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.