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Percoll is a reagent consisting of colloidal silica particles used in cell biology and other laboratory settings. It was first formulated by Pertoft and colleagues, [2] and commercialized by Pharmacia Fine Chemicals. [3] Percoll is used for the isolation of cells, organelles, or viruses by density centrifugation.
Some density-gradient work for purifying cells and organelles can be carried out in swinging-bucket rotors, or in the case of Percoll gradients in fixed-angle rotors. [9] High-speed or superspeed centrifuges can handle larger sample volumes, from a few tens of millilitres to several litres.
Using the example of a separation of eukaryotic organelles from intact cells, the cell must first be lysed and homogenized (ideally by a gentle technique, such as Dounce homogenization; harsher techniques or over homogenization will lead to a lower proportion of intact organelles). Once the crude organelle extract is obtained, it may be ...
It is defined to be the ratio of the rate of advection of a physical quantity by the flow to the rate of diffusion of the same quantity driven by an appropriate gradient. In the context of species or mass transfer, the Péclet number is the product of the Reynolds number and the Schmidt number (Re × Sc).
In Cartesian coordinates, the divergence of a continuously differentiable vector field = + + is the scalar-valued function: = = (, , ) (, , ) = + +.. As the name implies, the divergence is a (local) measure of the degree to which vectors in the field diverge.
The gradient of a function is obtained by raising the index of the differential , whose components are given by: =; =; =, = = The divergence of a vector field with components is
An example of such a product is the "Accuspin System Histopaque-1077" sold by Sigma Aldrich. The cost of this product is significantly higher than basic Ficoll and Ficoll-Paque products. It is also possible to have the Ficoll-Paque separating system included in the Vacutainer used to collect the blood.
The transport rate is usually estimated using the equations of Stokes flow, where the fluid velocity is obtained by equating the stress gradient to the viscous dissipation. A surface tension is a force per unit length, so the resulting stress must scale as Δ γ / L {\displaystyle \Delta \gamma /L} , while the viscous stress scales as μ u / L ...