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MCF-7 cells. MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old woman. [1] MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. [2] The Michigan Cancer Foundation is now known as the Barbara Ann Karmanos Cancer ...
If accepted it becomes a final decision and published semi-annually on the website and WHO Drug Information and implemented in the annual print/on-line ACT/DDD Index on January 1. [ 18 ] Changes to existing ATC/DDD follow a similar process to become temporary codes and if accepted become a final decision as ATC/DDD alterations.
ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development. Established in 1925 to serve as a national center for depositing and distributing microbiological specimens, ATCC has since grown to ...
The E-SCREEN cell proliferation assay is performed with the human MCF-7 breast cancer cell line, an established estrogenic cell line that endogenously expresses ERα. [ 1 ] [ 3 ] [ 4 ] Human MCF-7 are cultivated in Dulbecco’s modified Eagle’s medium (DMEM) with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 ...
CIWMB California Integrated Waste Management Board; CL:AIRE Contaminated Land: Application In Real Environments CLAIRE; CLEA Contaminated Land Exposure Assessment; CLO Compost-like output; CLR Contaminated Land Register; CNEA Clean Neighbourhoods and Environment Act; COD Chemical oxygen demand or certificate of destruction
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. [2] This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.
A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4). [ 1 ] Now there are McFarland standards prepared from suspensions of latex particles, which lengthens the shelf life and stability of the suspensions.
Houston Air Route Traffic Control Center, United States. The United States Federal Aviation Administration (FAA) defines an ARTCC as: [a] facility established to provide air traffic control service to aircraft operating on IFR flight plans within controlled airspace, principally during the en-route phase of flight.