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TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher Kary Mullis at Cetus Corporation, [ 1 ] and the technology was subsequently developed by Hoffmann-La Roche for diagnostic assays and by Applied Biosystems (now part of Thermo Fisher ...
The well position is also standardized, but only for 96- , 384-, and 1536-well plates. These are generally well followed by manufacturers: Well Positions [16] [17] 96-well plates have a 9 mm well-to-well spacing, 384-wells a 4.5 mm spacing, and 1536-wells a 2.25 mm spacing. A notable characteristic is that the well array is symmetrical when the ...
I designed the figure from an overlay of a 384-well plate manufacturer technical drawing. Designed in Inkscape. The Inkscape "Tiled clone" tool where the percentage row/column shift was calculated by (well spacing/well width-1.00)*100 (in this case, 12.5%), was essential to producing regularly spaced wells.
Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery , [ 1 ] bioassay validation, quality control and manufacturing processes in the pharmaceutical and ...
The importance of the high-precision surface plate was first recognised by Henry Maudslay around 1800. He originated the systems of scraping a cast-iron plate to flatness, rubbing marking blue between pairs of plates to highlight imperfections, and of working plates in sets of three to guarantee flatness by avoiding matching concave and convex ...
[7] [10] Plate length: Depending on the scale of the system, total plate lengths can vary, however, the plate length should allow for the plates to rise 125 mm above the top water level, with 1.5 m of space left below the plates at the bottom of the clarifier for collection of sludge. [7] Most plates have a length of 1–2 m. [14]
In general well heads are five nominal ratings of wellheads: 2, 3, 5, 10 and 15 (×1000) psi working pressure. They have an operating temperature range of −50 to +250 degrees Fahrenheit. They are used in conjunction with ring type seal gaskets. In general the yield strength of the materials range from 36000 to 75000 psi.
Most current battery holders are made with polypropylene or nylon bodies rated for 80–100 °C (176–212 °F). Lithium coin cell holders are made with high temperature PBT, nylon or LCP bodies because they normally are circuit board mounted and require wave soldering at 180–240 °C (356–464 °F) or reflow soldering at 230–300 °C (446 ...
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