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Biofluorescence is fluorescence exhibited by a living organism: part of the organism absorbs light or other radiation at one wavelength and emits visible light at another, usually longer. The absorbed radiation is often blue or ultraviolet , while the light emitted is typically green, red, or anything in between.
Biologically functional fluorescence is found in the photic zone, where there is not only enough light to cause fluorescence, but enough light for other organisms to detect it. [36] The visual field in the photic zone is naturally blue, so colors of fluorescence can be detected as bright reds, oranges, yellows, and greens.
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
When a certain molecule absorbs light, the energy of the molecule is briefly raised to a higher excited state. The subsequent return to ground state results in emission of fluorescent light that can be detected and measured. The emitted light, resulting from the absorbed photon of energy hv, has a specific wavelength. It is important to know ...
Laundry detergent fluorescing under ultraviolet light. Optical brighteners, optical brightening agents (OBAs), fluorescent brightening agents (FBAs), or fluorescent whitening agents (FWAs), are chemical compounds that absorb light in the ultraviolet and violet region (usually 340-370 nm) of the electromagnetic spectrum, and re-emit light in the blue region (typically 420-470 nm) through the ...
It happens because of something called the Rayleigh effect, or Rayleigh scattering, named after a British scientist who first wrote about it in 1871. Bands of vivid blue, pink and orange light are ...
As an oculofacial plastic surgeon, I've seen all kinds of different ways that wearing a mask can impact the under-eye area, and regularly get questions about what's going on.
In pseudofluorescence, non-fluorescent light is imaged. This occurs when blue light reflected from the retina passes through the filter. This is generally a problem with older filters, and annual replacement of these filters is recommended. In autofluorescence, fluorescence from the eye occurs without injection of the dye.