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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
A close up of an inoculation needle. An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. [1] [full citation needed] It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. [1]
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...
Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter: McCartney's bottle or Flat medical bottle
The term "inoculation" is also used more generally to refer to intentionally depositing microbes into any growth medium, as into a Petri dish used to culture the microbe, or into food ingredients for making cultured foods such as yoghurt and fermented beverages such as beer and wine.
In microbiology, the multiplicity of infection or MOI is the ratio of agents (e.g. phage or more generally virus, bacteria) to infection targets (e.g. cell).For example, when referring to a group of cells inoculated with virus particles, the MOI is the ratio of the number of virus particles to the number of target cells present in a defined space.
Dr. Schreiber of San Augustine giving a typhoid inoculation at a rural school, San Augustine County, Texas.Transfer from U.S. Office of War Information, 1944.. Immunization, or immunisation, is the process by which an individual's immune system becomes fortified against an infectious agent (known as the immunogen).