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NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence. [2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR ...
OLIGO Primer Analysis Software is a software for DNA primer design. [ 1 ] [ 2 ] The first paper describing this software was published in 1989. [ 3 ] The program is a real time PCR primer and probe search and analysis tool.
The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources. [7] [8] [9]
Primer Premier is a bioinformatics software used for various PCR applications. It supports the design of degenerate primers for amplifying a related set of nucleotide sequences for the detection of common traits amongst organisms, as well as to determine heredity. [1] The software also designs tagged and nested primers for multiplex PCR ...
CSS HTML Validator (previously named CSE HTML Validator) is an HTML editor and CSS editor for Microsoft Windows (and Linux and other Unix-like operating systems when used with Wine) that helps web developers create syntactically correct and accessible HTML/HTML5, XHTML, and CSS documents by locating errors, potential problems like browser compatibility issues, and common mistakes.
Get the tools you need to help boost internet speed, send email safely and security from any device, find lost computer files and folders and monitor your credit.
Primer extension can be used to determine the start site of transcription (the end site cannot be determined by this method) by which its sequence is known. This technique requires a radiolabelled primer (usually 20 - 50 nucleotides in length) which is complementary to a region near the 3' end of the mRNA.
The effect of a checksum algorithm that yields an n-bit checksum is to map each m-bit message to a corner of a larger hypercube, with dimension m + n. The 2 m + n corners of this hypercube represent all possible received messages. The valid received messages (those that have the correct checksum) comprise a smaller set, with only 2 m corners.