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Beacon Designer program has built-in support for homology avoidance using the standard web BLAST service or the set-up of a desktop BLAST feature for the identification of regions that are significantly cross-homologous with the target gene sequence. The software designs real-time PCR primers and probes for complex differential gene expression ...
OLIGO Primer Analysis Software is a software for DNA primer design. [1] [2] The first paper describing this software was published in 1989. [3]The program is a real time PCR primer and probe search and analysis tool.
Computer simulations of theoretical PCR results (Electronic PCR) may be performed to assist in primer design. [ 14 ] Touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers will avoid amplifying nonspecific sequences.
The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources. [7] [8] [9]
Primer Premier is a bioinformatics software used for various PCR applications. It supports the design of degenerate primers for amplifying a related set of nucleotide sequences for the detection of common traits amongst organisms, as well as to determine heredity. [1] The software also designs tagged and nested primers for multiplex PCR ...
Multiple sequence alignment: align sequences of DNA, RNA, [2] or amino acids. [3] Genome alignment: find and align the syntenic regions of multiple genomes. Oligonucleotide design: [4] primer design [5] [6] for polymerase chain reaction (PCR), probe design for fluorescence in situ hybridization (FISH) [7] or DNA microarrays. [8]
Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Pieter Vos, [ 1 ] AFLP uses restriction enzymes to digest genomic DNA , followed by ligation of adaptors to the sticky ends of the restriction ...
Ultra-fast sequence analysis tool: Both: Edgar, R. C. (2010). "Search and clustering orders of magnitude faster than BLAST". Bioinformatics. 26 (19): 2460– 2461. doi: 10.1093/bioinformatics/btq461. PMID 20709691. publication: 2010 OSWALD OpenCL Smith-Waterman on Altera's FPGA for Large Protein Databases Protein