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An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
Staphylococcus epidermidis, 1000 magnification under bright field microscopy. Staphylococcus epidermidis is a very hardy microorganism, consisting of nonmotile, Gram-positive cocci, arranged in grape-like clusters. It forms white, raised, cohesive colonies about 1–2 mm in diameter after overnight incubation, and is not hemolytic on blood agar ...
Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): [1] 0.5% peptone - this provides organic nitrogen; 0.3% beef extract/yeast extract - the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts; 1.5% agar - this ...
For example, if a microbiologist observes colonies that resemble a Staphylococcus species, they may perform a catalase test to confirm that it belongs to the genus Staphylococcus, and a coagulase test to determine whether it is a coagulase-negative staphylococcus or a more pathogenic species, such as S. aureus. [3]: 101 [8]: 203
If an organism does not induce hemolysis, the agar under and around the colony is unchanged and the organism is called non-hemolytic or said to display gamma-hemolysis (γ-hemolysis). Enterococcus faecalis (formerly called "Group D Strep"), Staphylococcus saprophyticus, and Staphylococcus epidermidis display gamma-hemolysis.
Vogel–Johnson agar is a type of agar growth medium selective for coagulase-positive staphylococci. It is used to isolate Staphylococcus aureus from clinical specimens and food. It was first described by Vogel and Johnson, who modified the Tellurite Glycine Agar recipe by Zebovitz et al. by doubling the mannitol concentration to 1% (w/v) and ...
Staphylococcus growth in Baird-Parker (BP) agar.. Baird-Parker agar is a type of agar used for the selective isolation of gram-positive Staphylococci species. [1] It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of Staphylococci. [1]
The spread plate method wherein the sample (in a small volume) is spread across the surface of a nutrient agar plate and allowed to dry before incubation for counting. [11] The membrane filter method wherein the sample is filtered through a membrane filter, then the filter placed on the surface of a nutrient agar plate.