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Automatic analysis of urine test strips using automated urine test strip analysers is a well-established practice in modern-day urinalysis. They can measure calcium , blood, glucose, bilirubin, urobilinogen, ketones, leukocytes, creatinine , microalbumin , pH, ascorbic acid and protein.
Test for ketonuria using Bayer Ketostix reagent strips. Urine testing is the most common method of testing for ketones. Urine test strips utilize a nitroprusside reaction with acetoacetate to give a semi-quantitative measure based on color change of the strip. Although beta-hydroxybutyrate is the predominant circulating ketone, urine test ...
Ketones occur in three forms in the body: beta-hydroxybutyrate (BHB), acetone and acetoacetate. Test strips use sodium nitroprusside to detect acetoacetate, and those with a glycine additive can detect acetone; however, none detect BHB. The reaction of ketones with sodium nitroprusside in an alkaline medium turns the test pad purple.
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It is seen in conditions in which the body produces excess ketones as an indication that it is using an alternative source of energy. It is seen during starvation or more commonly in type 1 diabetes mellitus. Production of ketone bodies is a normal response to a shortage of glucose, meant to provide an alternate source of fuel from fatty acids.
This is performed using ketone test strips containing nitroprusside, which change colour from buff-pink to maroon in the presence of acetoacetate (one of the three ketone bodies). [47] A short-lived increase in seizure frequency may occur during illness or if ketone levels fluctuate.