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The California mastitis test (CMT) is a simple cow-side indicator of the somatic cell count of milk. It operates by disrupting the cell membrane of any cells present in the milk sample, allowing the DNA in those cells to react with the test reagent, forming a gel. [1] It provides a useful technique for detecting subclinical cases of mastitis.
A plastic paddle used in the California mastitis test. Cattle affected by mastitis can be detected by examining the udder for inflammation and swelling, or by observing the consistency of the milk, which will often develop clots or change color when a cow is infected. [13]
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This test can be easily done in clinical practice. The flow or milk from the inflamed nipple is placed on a sterile pad. Budin's sign is positive if the milk is mixed with pus (brown, yellow or bloody traces). It is performed in cases of suspected mastitis and to differentiate between lymphangitis of the breast and infectious (often bacterial ...
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The presence of Corynebacterium in granulomatous mastitis was first reported in 1996. [14] Since then multiple reports have confirmed the presence of this genus in granulomatous mastitis. [15] [16] [17] The most commonly isolated species is Corynebacterium kroppenstedtii. A selective medium for the isolation of this species has been described. [18]