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C. canimorsus displays enhanced growth in high concentrations of carbon dioxide, so culturing the bacteria in candle extinction jars or carbon dioxide incubators is necessary. [4] To diagnose this bacillus, certain reactions may be tested. The bacterium should test positive for catalase and oxidase, arginine dihydrolase, maltose, and lactose.
The equation for this reaction is: carbon disulfide reacts with nitric oxide, which releases carbon dioxide, nitrogen and sulfur, which is then deposited on the walls of the cylinder and which is very inconvenient to wash off. The reaction is very beautiful, during which a bright flash occurs, and the so-called dog barking.
The air in the incubator was kept at 37 degrees Celsius, the same temperature as the human body, and the incubator maintained the atmospheric carbon dioxide and nitrogen levels necessary to promote cell growth. At this time, incubators also began to be used in genetic engineering. Scientists could create biologically essential proteins, such as ...
The components of the GasPak envelope along with the addition of water, allow for the start of the reaction that takes place in the catalyst chamber. The sealed anaerobic jar is then incubated at a desired temperature to allow growth of the bacteria. In the above figure for example, the incubation occurred at 100 °F (38 °C).
The candle's flame burns until extinguished by oxygen deprivation, creating a carbon dioxide-rich, oxygen-poor atmosphere. [ 5 ] Newer oxystat bioreactor methods allow for more precise control of gas levels in the microaerobic environment, using a probe to measure the oxygen concentration or redox potential in real time. [ 6 ]
The phase-change incubator is a low-cost, low-maintenance incubator that tests for microorganisms in water supplies. It uses small balls containing a chemical compound that, when heated and then kept insulated, will stay at 37 °C (approx. 99 °F) for 24 hours.