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Giuseppe Bertani (October 23, 1923–April 7, 2015) was a microbial geneticist who did work with Drosophila melanogaster, mutation rates in E. coli, and spent most of his career working with phages, specifically phage P2.
Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani , intended LB to stand for lysogeny broth , [ 1 ] but LB has also come to colloquially mean Luria broth , Lennox broth , life broth or Luria –Bertani medium . [ 2 ]
Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms. [2] It provides a source of amino acids for the growing bacteria .
Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. This nutrient-rich microbial broth contains peptides , amino acids , water soluble vitamins and glucose in a low-salt formulation.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
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Nutrient broth has the same composition, but lacks agar. [2] [3] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.
A lysogen or lysogenic bacteria is a bacterial cell that can produce and transfer the ability to produce a phage. [1] A prophage is either integrated into the host bacteria's chromosome or more rarely exists as a stable plasmid within the host cell.