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The simplest version simply dispenses an allotted volume of liquid from a motorized pipette or syringe; more complicated machines can also manipulate the position of the dispensers and containers (often a Cartesian coordinate robot, and/or integrate additional laboratory devices, such as centrifuges, microplate readers, heat sealers, heater/shakers, bar code readers, spectrophotometric devices ...
Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid ...
Release the pipette knob slowly to the ready position. Do not remove the tip from the solution. Repeat this process (step 3 and 4) to rinse the tip until the interior wall of the tip is clear. Remove the tip from the solution. Press the pipette knob to the second stop, and completely empty the tip. Release the pipette knob to the ready position.
These pipettes operate by piston-driven air displacement. A vacuum is generated by the vertical travel of a metallic or ceramic piston within an airtight sleeve. The upward movement of the piston, driven by the depression of the plunger, creates a vacuum in the space left vacant by the piston.
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette.The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: [1] as it reduces the risk of splashing, foam or bubble formation.
A graduated pipette is a pipette with its volume, in increments, marked along the tube. It is used to accurately measure and transfer a volume of liquid from one container to another. [ 1 ] It is made from plastic or glass tubes and has a tapered tip.
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