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A DNA construct is an artificially-designed segment of DNA borne on a vector that can be used to incorporate genetic material into a target tissue or cell. [1] A DNA construct contains a DNA insert, called a transgene, delivered via a transformation vector which allows the insert sequence to be replicated and/or expressed in the target cell.
The vector itself generally carries a DNA sequence that consists of an insert (in this case the transgene) and a larger sequence that serves as the "backbone" of the vector. The purpose of a vector which transfers genetic information to another cell is typically to isolate, multiply, or express the insert in the target cell.
In the natural sciences, a vector quantity (also known as a vector physical quantity, physical vector, or simply vector) is a vector-valued physical quantity. [ 1 ] [ 2 ] It is typically formulated as the product of a unit of measurement and a vector numerical value ( unitless ), often a Euclidean vector with magnitude and direction .
The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. [1]
The Phusion DNA polymerase fills in any gaps where the fragments anneal. Taq DNA ligase repairs the nicks on both DNA strands. Because the T5 exonuclease is heat labile, it is inactivated at 50 °C after the initial chew back step. The product is thus stable, and the fragments assembled in the desired order.
An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene.
The final column lists some special properties that some of the quantities have, such as their scaling behavior (i.e. whether the quantity is intensive or extensive), their transformation properties (i.e. whether the quantity is a scalar, vector, matrix or tensor), and whether the quantity is conserved.
In the natural sciences, a vector quantity (also known as a vector physical quantity, physical vector, or simply vector) is a vector-valued physical quantity. [9] [10] It is typically formulated as the product of a unit of measurement and a vector numerical value , often a Euclidean vector with magnitude and direction.