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  2. Oil immersion - Wikipedia

    en.wikipedia.org/wiki/Oil_immersion

    Oil-immersion objective lenses look superficially identical to non-oil-immersion lenses. In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical ...

  3. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The actual power or magnification of a compound optical microscope is the product of the powers of the eyepiece and the objective lens. For example a 10x eyepiece magnification and a 100x objective lens magnification gives a total magnification of 1,000×.

  4. Objective (optics) - Wikipedia

    en.wikipedia.org/wiki/Objective_(optics)

    Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...

  5. High-power field - Wikipedia

    en.wikipedia.org/wiki/High-power_field

    The area provides a reference unit, for example in reference ranges for urine tests. [3]Used for grading of soft tissue tumors: Grading, usually on a scale of I to III, is based on the degree of differentiation, the average number of mitoses per high-power field, cellularity, pleomorphism, and an estimate of the extent of necrosis (presumably a reflection of rate of growth).

  6. Water immersion objective - Wikipedia

    en.wikipedia.org/wiki/Water_immersion_objective

    In light microscopy, a water immersion objective is a specially designed objective lens used to increase the resolution of the microscope. This is achieved by immersing both the lens and the specimen in water which has a higher refractive index than air, thereby increasing the numerical aperture of the objective lens.

  7. Condenser (optics) - Wikipedia

    en.wikipedia.org/wiki/Condenser_(optics)

    The maximum NA is limited by the refractive index of the medium between the lens and the sample. As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the ...

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