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The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
Primers for heat-shrinkable sleeves work in the same manner as an FBE primer does when it is specified on 3-layer polyolefin pipeline coatings and is typically applied between 150 μm and 300 μm thick. Usually, the primer of heat shrinkable sleeve is two components non-solvent Epoxy, one is primer base and the other is curing agent.
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NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence. [2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm.
A cycloidal drive or cycloidal speed reducer is a mechanism for reducing the speed of an input shaft by a certain ratio. Cycloidal speed reducers are capable of relatively high ratios in compact sizes with very low backlash. [1] The input shaft drives an eccentric bearing that in turn drives the cycloidal disc in an eccentric, cycloidal motion ...
A propeller speed reduction unit is a gearbox or a belt and pulley device used to reduce the output revolutions per minute (rpm) from the higher input rpm of the powerplant. [1] This allows the use of small displacement internal combustion engines to turn aircraft propellers within an efficient speed range.