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Transient expression, more frequently referred to "transient gene expression", is the temporary expression of genes that are expressed for a short time after nucleic acid, most frequently plasmid DNA encoding an expression cassette, has been introduced into eukaryotic cells with a chemical delivery agent like calcium phosphate (CaPi) or polyethyleneimine (PEI). [1]
An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene.
An inducible gene is a gene whose expression is either responsive to environmental change or dependent on the position in the cell cycle. Any step of gene expression may be modulated, from the DNA-RNA transcription step to post-translational modification of a protein. The stability of the final gene product, whether it is RNA or protein, also ...
The host organism can be a bacterium, yeast, mammalian cell, or plant cell. This host is called the " expression system ". Homologous expression , on the other hand, refers to the overexpression of a gene in a system from where it originates.
Non-lytic insect cell expression is an alternative to the lytic baculovirus expression system. In non-lytic expression, vectors are transiently or stably transfected into the chromosomal DNA of insect cells for subsequent gene expression. [23] [24] This is followed by selection and screening of recombinant clones. [25]
An expression cassette comprises three components: a promoter sequence, an open reading frame, and a 3' untranslated region that, in eukaryotes, usually contains a polyadenylation site. [ 4 ] Different expression cassettes can be transfected into different organisms including bacteria, yeast, plants, and mammalian cells as long as the correct ...
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