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Adjustment knobs move the stage up and down with separate adjustment for coarse and fine focusing. The same controls enable the microscope to adjust to specimens of different thickness. In older designs of microscopes, the focus adjustment wheels move the microscope tube up or down relative to the stand and had a fixed stage. [citation needed]
The focus of the first lens is traditionally about 2mm away from the plane face coinciding with the sample plane. A pinhole cap can be used to align the optical axis of the condenser with that of the microscope. The Abbe condenser is still the basis for most modern light microscope condenser designs, even though its optical performance is poor.
The stage of an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment.
A traveling microscope. E—eyepiece, O—objective, K—knob for focusing, V—vernier, R—rails, S—screw for fine position adjustment. A travelling microscope is an instrument for measuring length with a resolution typically in the order of 0.01mm.
Two control knobs for a heating/cooling system. The left knob controls the temperature while the right controls the fan speed. A control knob is a rotary device used to provide manual input adjustments to a mechanical/electrical system when grasped and turned by a human operator, so that differing extent of knob rotation corresponds to different desired input.
Zoom lenses (sometimes referred to as "true" zoom) are ideally parfocal, in that focus is maintained as the lens is zoomed (i.e., focal length and magnification changed), which is convenient and has the advantage of allowing more accurate focusing at maximal focal length then zooming back to a shorter focal length to compose the image.
The resolution of a microscope is defined as the minimum separation needed between two objects under examination in order for the microscope to discern them as separate objects. This minimum distance is labelled δ. If two objects are separated by a distance shorter than δ, they will appear as a single object in the microscope.
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.