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CAMP test is shown at bottom left. The CAMP test (Christie–Atkins–Munch-Petersen) is a test to identify group B β-hemolytic streptococci (Streptococcus agalactiae) [1] [2] based on their formation of a substance, CAMP factor, [3] that enlarges the area of hemolysis formed by the β-hemolysin elaborated from Staphylococcus aureus.
This phenomenon is the mechanism behind the CAMP test, [2] a test that was historically used for the identification of Streptococcus agalactiae and Listeria monocytogenes. [3] A modified version of this test called the reverse CAMP test, utilizing S. agalactiae instead of S. aureus, can also be used to identify Clostridium perfringens.
A rapid strep test may assist a clinician in deciding whether to prescribe an antibiotic to a person with pharyngitis, a common infection of the throat. [1] Viral infections are responsible for the majority of pharyngitis, but a significant proportion (20% to 40% in children and 5% to 15% in adults) is caused by bacterial infection. [2]
Streptococcus pyogenes, or GAS, displays beta hemolysis. Some weakly beta-hemolytic species cause intense hemolysis when grown together with a strain of Staphylococcus. This is called the CAMP test. Streptococcus agalactiae displays this property. Clostridium perfringens can be identified presumptively with this test.
have had group B streptococcal colonization, bacteriuria, or infection in a previous pregnancy, and have not had a negative test for group B streptococcus by enrichment culture or PCR on a rectovaginal swab sample collected between 35 and 37 weeks' gestation or 3-5 weeks before the anticipated delivery date in the current pregnancy or
The reverse CAMP test utilizes the synergetic hemolytic abilities of the CAMP factor produced by Streptococcus agalactiae with the α-toxin produced by Clostridium perfringens. Streaking these two organisms perpendicular to each other on a blood agar plate will yield a “bow-tie” clearing of the blood agar by the hemolytic capabilities of ...
Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England setting. Main Streptococcus groups are included as "Strep." at bottom left. Diagnosis is by a swab of the affected area for laboratory testing.
For example, when carbohydrates are fermented, the pH within the well decreases and that is indicated by a change in the color of the pH indicator. All test results are compiled to obtain a profile number, which is then compared with profile numbers in a commercial codebook (or online) to determine the identification of the bacterial species.