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Genetic testing, such as via polymerase chain reaction (PCR), DNA microarray, and loop-mediated isothermal amplification, may be used to detect whether bacteria possess genes which confer antibiotic resistance. [9] [23] An example is the use of PCR to detect the mecA gene for beta-lactam resistant Staphylococcus aureus. [9]
The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the ...
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...
An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. [1] [full citation needed] It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. [1] A standard reusable inoculation needle is made from nichrome or platinum wire ...
Wet each disk with about four inoculating loops of deionized water. Use a loop to aseptically transfer a large mass of pure bacteria to the disk. Observe the disk for up to three minutes. If the area of inoculation turns dark-blue to maroon to almost black, then the result is positive.
a 26 gauge needle bent twice used for incising the anterior capsule of the lens in lens extraction Wire vectis: a loop of wire attached to a stack used to extract cataract affected lenses Irrigating vectis: a small hollow instrument with a used to introduce fluid into the anterior chamber to raise its pressure to aid cataract extraction [2] Canula
Sulphide Indole Motility (SIM) medium is a bacterial growth medium which tests for the ability to reduce sulfates, the ability to produce indoles, and motility. [1] This combination of challenges in one mixture is convenient and commercially available in stab tubes.