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Colonial morphology of various specimens of Pseudomonas aeruginosa, including mucoid types. In microbiology, colonial morphology refers to the visual appearance of bacterial or fungal colonies on an agar plate. Examining colonial morphology is the first step in the identification of an unknown microbe.
E. coli on EMB agar. Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.
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Cetrimide also enhances the production of Pseudomonas pigments such as pyocyanin and pyoverdine, which show a characteristic blue-green and yellow-green colour, respectively. [3] [4] Cetrimide agar is widely used in the examination of cosmetics, pharmaceuticals and clinical specimens to test for the presence of Pseudomonas aeruginosa. [1]
The genome of Pseudomonas aeruginosa consists of a relatively large circular chromosome (5.5–6.8 Mb) that carries between 5,500 and 6,000 open reading frames, and sometimes plasmids of various sizes depending on the strain. [11] Comparison of 389 genomes from different P. aeruginosa strains showed that just 17.5% is shared.
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
Pseudomonas infection refers to a disease caused by one of the species of the genus Pseudomonas. P. aeruginosa is a germ found in the environment and it is an opportunistic human pathogen most commonly infecting immunocompromised patients, such as those with cancer, diabetes, cystic fibrosis, [1] severe burns, AIDS, [2] or people who are very ...
Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar.