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Ellman's original 1959 publication estimated the molar extinction at 13,600 M −1 cm −1, and this value can be found in some modern applications of the method despite improved determinations. [6] Commercial DTNB may not be completely pure, so may require recrystallization to obtain completely accurate and reproducible results.
tert-Butanesulfinamide (also known as 2-methyl-2-propanesulfinamide or Ellman's sulfinamide) is an organosulfur compound and a member of the class of sulfinamides. Both enantiomeric forms are commercially available and are used in asymmetric synthesis as chiral auxiliaries , often as chiral ammonia equivalents for the synthesis of amines .
Extinction coefficient refers to several different measures of the absorption of light in a medium: Attenuation coefficient , sometimes called "extinction coefficient" in meteorology or climatology Mass extinction coefficient , how strongly a substance absorbs light at a given wavelength, per mass density
This is the talk page for discussing improvements to the Ellman's reagent article. ... "Prediction of molar extinction coefficients of proteins and peptides using UV ...
A. R. Forouhi and I. Bloomer deduced dispersion equations for the refractive index, n, and extinction coefficient, k, which were published in 1986 [1] and 1988. [2] The 1986 publication relates to amorphous materials, while the 1988 publication relates to crystalline.
The molar absorption coefficient is also known as the molar extinction coefficient and molar absorptivity, but the use of these alternative terms has been discouraged by the IUPAC. [ 2 ] [ 3 ] Beer–Lambert law
The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
[1] [2] The amount of product formed is limited by this reagent, since the reaction cannot continue without it. If one or more other reagents are present in excess of the quantities required to react with the limiting reagent, they are described as excess reagents or excess reactants (sometimes abbreviated as "xs"), or to be in abundance. [3]