Search results
Results From The WOW.Com Content Network
Restriction digest is most commonly used as part of the process of the molecular cloning of DNA fragment into a vector (such as a cloning vector or an expression vector).The vector typically contains a multiple cloning site where many restriction site may be found, and a foreign piece of DNA may be inserted into the vector by first cutting the restriction sites in the vector as well the DNA ...
The density of RAD tags in a genome depends on the restriction enzyme used during the isolation process. [5] There are other restriction site marker techniques, like RFLP or amplified fragment length polymorphism (AFLP), which use fragment length polymorphism caused by different restriction sites, for the distinction of genetic polymorphism ...
RRBS uniquely uses a specific restriction enzyme to enrich for CpGs. MspI digestion, or any restriction enzyme that recognizes CpG's and cuts them, produces only fragments with CG’s at the end. [2] This approach enriches for CpG regions of the genome, so it can decrease the amount of sequencing required as well as decrease the cost. [2]
The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites. Combined Bisulfite Restriction Analysis (or COBRA) is a molecular biology technique that allows for the sensitive quantification of DNA methylation levels at a specific genomic locus on a DNA sequence in a small sample of genomic DNA. [1]
In molecular biology, XhoI is a type II restriction enzymes that recognise the double-stranded DNA sequence CTCGAG and cleaves after C-1. [1] Type II restriction endonucleases are components of prokaryotic DNA restriction-modification mechanisms that protect the organism against invading foreign DNA.
This is the main advantage of OE-PCR and other long-homology based cloning methods such as Gibson assembly, which overcome the limitations of traditional restriction enzyme digestion and ligation cloning methods. [3] Assembly of custom DNA sequences with OE-PCR consists on three main steps.
In molecular biology, REBASE is a database of information about restriction enzymes and DNA methyltransferases. [1] REBASE contains an extensive set of references, sites of recognition and cleavage, sequences and structures. It also contains information on the commercial availability of each enzyme.
Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel, 3 volt/cm, stained with ethidium bromide. The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted.