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A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope. Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining technique and the eosin counterstain to haematoxylin in the H&E stain . [ 1 ]
Eosin is most often used as a counterstain to haematoxylin, imparting a pink or red colour to cytoplasmic material, cell membranes, and some extracellular structures. It also imparts a strong red colour to red blood cells. Eosin may also be used as a counterstain in some variants of Gram staining, and in many other protocols.
Hindustani, the lingua franca of Northern India and Pakistan, has two standardised registers: Hindi and Urdu.Grammatical differences between the two standards are minor but each uses its own script: Hindi uses Devanagari while Urdu uses an extended form of the Perso-Arabic script, typically in the Nastaʿlīq style.
Counterstain, which is usually positively charged safranin or basic fuchsine, is applied last to give decolorized gram-negative bacteria a pink or red color. [3] [20] Both gram-positive bacteria and gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on gram-positive bacteria because of the darker crystal ...
Hindustani, also known as Hindi-Urdu, like all Indo-Aryan languages, has a core base of Sanskrit-derived vocabulary, which it gained through Prakrit. [1] As such the standardized registers of the Hindustani language (Hindi-Urdu) share a common vocabulary, especially on the colloquial level. [ 2 ]
The Hindustani language is the language of Hindustan and the lingua franca of the northern Indian subcontinent. [22] Hindustani derives from the Old Hindi language of Western Uttar Pradesh and Delhi areas. Its literary standard forms—Modern Standard Hindi and Modern Standard Urdu—use different scripts.
Located in the Hindi Belt, the Central Zone includes the Dehlavi (Delhi) dialect (one of several called 'Khariboli') of the Hindustani language, the lingua franca of Northern India that is the basis of the Modern Standard Hindi and Standard Urdu literary standards. In regards to the Indo-Aryan language family, the coherence of this language ...
[1] Another factor in stain removal is the fact that stains can sometimes comprise two separate staining agents, which require separate forms of removal. A machine oil stain could also contain traces of metal, for example. [1] Also of concern is the color of the material that is stained.