Search results
Results From The WOW.Com Content Network
Palindrome of DNA structure A: Palindrome, B: Loop, C: Stem. A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule whereby reading in a certain direction (e.g. 5' to 3') on one strand is identical to the sequence in the same direction (e.g. 5' to 3') on the complementary strand. This definition of palindrome ...
The presences of a poly G sequence at the 5' end, the 3' end, or both; An internal palindrome sequence; GC dinucleotides contained within the internal palindrome; A partially PS-modified backbone; Class A ODN typically contain 7 to 10 PS-modified bases at one or both ends that resist degradation by nucleases and increase the longevity of the ODN.
The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site. [ 5 ] [ 6 ] Isoschizomers and neoschizomers : An isoschizomer is a restriction enzyme that recognizes the same sequence as another.
The intervening sequence of nucleotides between the initial sequence and the reverse complement can be any length including zero. For example, 5'---TTACGnnnnnn CGTAA---3' is an inverted repeat sequence. When the intervening length is zero, the composite sequence is a palindromic sequence. [2]
For example, there are two PstI recognition sites in the following DNA sequence fragment, starting at base 9 and 31 respectively. A recognition sequence is a specific sequence, usually very short (less than 10 bases). Depending on the degree of specificity of the protein, a DNA-binding protein can bind to more than one specific sequence.
Inverted repeat sequences on double-stranded DNA suggest the formation of cruciform structures. Cruciform DNA is a form of non-B DNA, or an alternative DNA structure. The formation of cruciform DNA requires the presence of palindromes called inverted repeat sequences. [1]
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg 2+ via hydrolysis. [1] HindIII restrictions process results in formation of overhanging palindromic sticky ends.
This E-box consists of the DNA sequence CANNTG, where N can be any nucleotide. [ 7 ] 1994: Harrison's [ 12 ] and Pabo's [ 13 ] groups crystallize bHLH proteins bound to E-boxes, demonstrating that the parallel 4-helix bundle motif loop orients the basic sequences to interact with specific nucleotides in the major groove of the E-box.