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An alternative definition says that an ORF is a sequence that has a length divisible by three and is bounded by stop codons. [1] [4] This more general definition can be useful in the context of transcriptomics and metagenomics, where a start or stop codon may not be present in the obtained sequences. Such an ORF corresponds to parts of a gene ...
The definition of a type 1 and type 2 sequence was first introduced by Vail et al. (1984). [4] Since they were hard to recognize, they were redefined in 1990 by Van Wagoner et al.. However even with this new definition, type 2 sequence boundaries were hard to recognize in the field due to their lack of subaerial exposure.
A sequence in biology is the one-dimensional ordering of monomers, covalently linked within a biopolymer; it is also referred to as the primary structure of a biological macromolecule. While it can refer to many different molecules, the term sequence is most often used to refer to a DNA sequence or a protein sequence .
The coding sequence (green) is bounded by untranslated regions at both the 5'-end (yellow) and the 3'-end (pink). Prior to export from the nucleus, a 5' cap (red) and a 3' poly(A) tail (black) are added to help stabilize the mRNA and prevent its degradation by ribonucleases. metabolism
The following DNA sequences illustrate pair double-stranded patterns. By convention, the top strand is written from the 5′-end to the 3′-end; thus, the bottom strand is written 3′ to 5′. A base-paired DNA sequence: ATCGATTGAGCTCTAGCG TAGCTAACTCGAGATCGC The corresponding RNA sequence, in which uracil is substituted for thymine in the RNA ...
However, it is sometimes desirable to sequence RNA molecules. While sequencing DNA gives a genetic profile of an organism, sequencing RNA reflects only the sequences that are actively expressed in the cells. To sequence RNA, the usual method is first to reverse transcribe the RNA extracted from the sample to generate cDNA fragments. This can ...
An infinite sequence of real numbers (in blue). This sequence is neither increasing, decreasing, convergent, nor Cauchy.It is, however, bounded. In mathematics, a sequence is an enumerated collection of objects in which repetitions are allowed and order matters.
The split gene theory is a theory of the origin of introns, long non-coding sequences in eukaryotic genes between the exons. [1] [2] [3] The theory holds that the randomness of primordial DNA sequences would only permit small (< 600bp) open reading frames (ORFs), and that important intron structures and regulatory sequences are derived from stop codons.