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If the stationary phase is non-polar, like C18-functionalized silica plates, it is called reverse-phase TLC. In this case, non-polar compounds move less and polar compounds move more. [citation needed] The solvent mixture will also be much more polar than in normal-phase TLC. [11]
Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [1] [2] [3] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the reversed ...
The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column. In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated. The mobile phase ...
Chiral thin-layer chromatography is a variant of liquid chromatography that is employed for the separation of enantiomers. It is necessary to use either It is necessary to use either a chiral stationary phase or
In this application, due to silica gel's polarity, non-polar components tend to elute before more polar ones, hence the name normal phase chromatography. However, when hydrophobic groups (such as C 18 groups) are attached to the silica gel then polar components elute first and the method is referred to as reverse phase chromatography.
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Even non-polar bonded silicas have been used with extremely high organic solvent composition, thanks to the exposed patches of silica in between the bonded ligands on the support, which can affect the interactions. [4] With that exception, HILIC phases can be grouped into five categories of neutral polar or ionic surfaces: [5]
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