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Peptide Mass Fingerprint search Identifies proteins from an uploaded peak list using a technique known as peptide mass fingerprinting. Sequence query Combines peptide mass data with amino acid sequence and composition information usually obtained from MS/MS tandem mass spectrometry data. Based on the peptide sequence tag approach. MS/MS Ion Search
A typical workflow of a peptide mass fingerprinting experiment. Peptide mass fingerprinting (PMF), also known as protein fingerprinting, is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. [1]
Protein sequence interpretation: a scheme new protein to be engineered in a yeast. It is often desirable to know the unordered amino acid composition of a protein prior to attempting to find the ordered sequence, as this knowledge can be used to facilitate the discovery of errors in the sequencing process or to distinguish between ambiguous results.
Point a is an ordinary point when functions p 1 (x) and p 0 (x) are analytic at x = a. Point a is a regular singular point if p 1 (x) has a pole up to order 1 at x = a and p 0 has a pole of order up to 2 at x = a. Otherwise point a is an irregular singular point.
For a peptide spectral library, to reach a maximal coverage is a long-term goal, even with the support of scientific community and ever-growing proteomic technologies. [ citation needed ] However, the optimization for a particular module of the peptide spectra library is a more manageable goal, e.g. the proteins in a particular organelle or ...
Hence, it is technically more correct to discuss singular points of a smooth mapping here rather than a singular point of a curve. The above definitions can be extended to cover implicit curves which are defined as the zero set f − 1 ( 0 ) {\displaystyle f^{-1}(0)} of a smooth function , and it is not necessary just to consider ...
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A peptide microarray is a planar slide with peptides spotted onto it or assembled directly on the surface by in-situ synthesis. Whereas peptides spotted can undergo quality controls that include mass spectrometer analysis and concentration normalization before spotting and result from a single synthetic batch, peptides synthesized directly on the surface may suffer from batch-to-batch ...