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Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for qPCR.
Housekeeping genes and proteins, including β-Actin, GAPDH, HPRT1, and RPLP1, are often used as internal controls in western blots because they are thought to be expressed constitutively, at the same levels, across experiments.
A loading control is a protein used as a control in a Western blotting experiment. Typically, loading controls are proteins with high and ubiquitous expression, such as beta-actin or GADPH. They are used to make sure that the protein has been loaded equally across all wells. [1] [2]
Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
The Šidák correction is derived by assuming that the individual tests are independent.Let the significance threshold for each test be ; then the probability that at least one of the tests is significant under this threshold is (1 - the probability that none of them are significant).
Tomiko Itooka, a 116-year-old Japanese woman who became the oldest living person in August 2024, died on Dec. 29, 2024, according to Guinness World Records.
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