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Golgi's method is a silver staining technique that is used to visualize nervous tissue under light microscopy. The method was discovered by Camillo Golgi, an Italian physician and scientist, who published the first picture made with the technique in 1873. [1]
Nervous tissue, also called neural tissue, is the main tissue component of the nervous system.The nervous system regulates and controls body functions and activity. It consists of two parts: the central nervous system (CNS) comprising the brain and spinal cord, and the peripheral nervous system (PNS) comprising the branching peripheral nerves.
White matter is the tissue through which messages pass between different areas of grey matter within the central nervous system. The white matter is white because of the fatty substance (myelin) that surrounds the nerve fibers (axons). This myelin is found in almost all long nerve fibers, and acts as an electrical insulation.
Some previous works used nerve cell (cellule nervose), as adopted in Camillo Golgi's 1873 paper on the discovery of the silver staining technique used to visualize nervous tissue under light microscopy.
An individual nerve consists of a number of fascicles, i.e. bundles of nerve fibers enclosed within a connective tissue sheath containing the different nerve fibers. Therefore, the tip of the microelectrode needs to be not only intraneural but also intrafascicular for a recording to be possible.
Grey matter, or gray matter in American English, is a major component of the central nervous system, consisting of neuronal cell bodies, neuropil (dendrites and unmyelinated axons), glial cells (astrocytes and oligodendrocytes), synapses, and capillaries.
Tactile corpuscles are encapsulated myelinated nerve endings, [3] surrounded by Schwann cells. [3] The encapsulation consists of flattened supportive cells arranged as horizontal lamellae surrounded by a connective tissue capsule. The corpuscle is 30–140 μm in length and 40–60 μm in diameter.
Such transport of molecules towards and away from the soma maintains critical cell functions. In case of neurons, the soma receives a large number of inhibitory synapses, [6] which can regulate the activity of these cells. It has also been shown that microglial processes constantly monitor neuronal functions through somatic junctions, and exert ...