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Deoxyribonuclease II (DNase II) is also known as acid deoxyribonuclease because it has optimal activity in the low pH environment of lysosomes where it is typically found in higher eukaryotes. Some forms of recombinant DNase II display a high level of activity in low pH in the absence of divalent metal ions, similar to eukaryotic DNase II. [7]
The method uses an enzyme, deoxyribonuclease (DNase, for short), to cut the radioactively end-labeled DNA, followed by gel electrophoresis to detect the resulting cleavage pattern. For example, the DNA fragment of interest may be PCR amplified using a 32 P 5' labeled primer, with the result being many DNA molecules with a radioactive label on ...
Deoxyribonuclease I (usually called DNase I), is an endonuclease of the DNase family coded by the human gene DNASE1. [5] DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide , yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average ...
Anti-Deoxyribonuclease B (anti-DNase B) titres are a quantitative measure of the presence of serologic antibodies obtained from patients suspected of having a recent group A (Beta-hemolytic) streptococcus bacteria infection, from Streptococcus pyogenes.
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In biochemistry, an endodeoxyribonuclease is a class of enzyme which is a type of deoxyribonuclease (a DNA cleaver), itself a type of endonuclease (a nucleotide cleaver). They catalyze cleavage of the phosphodiester bonds in DNA. They are classified with EC numbers 3.1.21 through 3.1.25. Examples include: DNA restriction enzymes
Deoxyribonuclease; External links. Exodeoxyribonucleases at the U.S. National Library of Medicine Medical Subject Headings (MeSH) This page was last edited on 11 ...
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg 2+ via hydrolysis. [1] HindIII restrictions process results in formation of overhanging palindromic sticky ends.