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FISSEQ combines the spatial context of RNA-FISH and the global transcriptome profiling of RNA-seq. [1] FISSEQ preserves the tissue allowing single molecule in situ RNA localization. The foundation of the method is a novel nucleic acid sequencing library construction method that stably cross-links cDNA amplicons within biological samples. [2]
The purpose of using RNA FISH is to detect target mRNA transcripts in cells, tissue sections, or even whole-mounts. [10] The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post-hybridization). The tissue preparation starts by collecting the appropriate tissue sections to perform RNA FISH.
Isopod fish parasites are mostly external and feed on blood. The larvae of the Gnathiidae family and adult cymothoidids have piercing and sucking mouthparts and clawed limbs adapted for clinging onto their hosts. [20] [21] Cymothoa exigua is a parasite of various marine fish. It causes the tongue of the fish to atrophy and takes its place in ...
Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...
These can be categorized into three groups; cestodes, nematodes and trematodes.Examples include: Acanthocephala; Ascariasis (roundworms); Cestoda (tapeworms) including: Taenia saginata (human beef tapeworm), Taenia solium (human pork tapeworm), Diphyllobothrium latum (fish tapeworm) and Echinococcosis (hydatid tapeworm)
The following is a list of aquarium diseases. Aquarium fish are often susceptible to numerous diseases, due to the artificially limited and concentrated environment. New fish can sometimes introduce diseases to aquaria, and these can be difficult to diagnose and treat. Most fish diseases are also aggravated when the fish is stressed.
56473 Ensembl ENSG00000134824 ENSMUSG00000024665 UniProt O95864 Q9Z0R9 RefSeq (mRNA) NM_001281501 NM_001281502 NM_004265 NM_019699 RefSeq (protein) NP_001268430 NP_001268431 NP_004256 NP_062673 Location (UCSC) Chr 11: 61.79 – 61.87 Mb Chr 19: 10.04 – 10.08 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse linoleoyl-CoA desaturase Identifiers EC no. 1.14.19.3 CAS no. 9014-34-0 ...
A test strip is a band/piece/strip of paper or other material used for biological testing. Specifically, test strip may refer to: Food testing strips; Glucose meter test strip; Lipolysis test strip; Urine test strip; Universal indicator pH test strips; It may also refer to: Teststrip, an art gallery in Auckland, New Zealand