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NP-40 (also known as Tergitol-type NP-40 and nonyl phenoxypolyethoxylethanol [1]) is a commercially available detergent with CAS Registry Number 9016-45-9. NP-40 is an ethoxylated nonylphenol for non-ionic surfactants and can act as emulsifier and demulsifier agent.
Nonidet P-40 is a nonionic, non-denaturing detergent. Its official IUPAC name is octylphenoxypolyethoxyethanol. Nonidet P-40 is sometimes abbreviated as NP-40, but should not be confused with a different detergent by the same name NP-40 , nonylphenoxypolyethoxyethanol of the Tergitol NP series of Dow Chemicals.
To make NPEs, manufacturers treat NP with ethylene oxide under basic conditions. [12] Since its discovery in 1940, nonylphenol production has increased exponentially, and between 100 and 500 million pounds of nonylphenol are produced globally every year, [ 12 ] [ 16 ] meeting the definition of High Production Volume Chemicals .
IGEPAL CA-630 has HLB of 13.4, similar to that of Triton X-100 (13.4) and thus belongs to the detergent range (HLB 13-15); this is significantly less than 17.8 of tergitol NP-40 or 16.7 of Polysorbate 20 (also known as Tween 20), which both belong in the solubilizer range (15-18) of HLB. [2] Ca-630 is completely miscible with water.
The solubilizing agent is NP-40, which can be replaced by other detergents at different concentrations. Since NP-40 is a nonionic detergent, this lysis buffer has a milder effect than RIPA buffer. It can be used when protein functions are to be retained with minimal disruption. [9] Recipe: [9] 150 mM NaCl; 1.0% Nonidet P-40 or Triton X-100; 50 ...
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). [1] [2] This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of ...
Nominal Pipe Size (NPS) is a North American set of standard sizes for pipes used for high or low pressures and temperatures. [1] " Nominal" refers to pipe in non-specific terms and identifies the diameter of the hole with a non-dimensional number (for example – 2-inch nominal steel pipe" consists of many varieties of steel pipe with the only criterion being a 2.375-inch (60.3 mm) outside ...
Each of the five models, the FNP-45, the FNP-40, the FNP-357, the FNP-9 and the compact FNP-9M (cancelled), are offered with the following features: Double/single action, Double-action only, or Single-action only (cancelled)