Search results
Results From The WOW.Com Content Network
Barfoed's test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu 2 O), which forms a brick-red precipitate. [1] [2] RCHO + 2Cu 2+ + 2H 2 O → RCOOH + Cu 2 O↓ + 4H + (Disaccharides may also react, but the reaction is much slower.)
Christen Thomsen Barfoed. Christen Thomsen Barfoed (16 June 1815 – 30 April 1889) was a Danish chemist credited with the development of a method to detect monosaccharide sugars in a solution, now known as the Barfoed's test. [1] [2] Barfoed is also credited with having introduced systematic chemical analyses in Danish agricultural sciences. [3]
It is advised to check the references for photos of reaction results. [1] Reagent testers might show the colour of the desired substance while not showing a different colour for a more dangerous additive. [2] For this reason it is essential to use multiple different tests to show all adulterants.
It is done in one step where the Bradford reagent is added to a test tube along with the sample. After mixing well, the mixture almost immediately changes to a blue color. When the dye binds to the proteins through a process that takes about 2 minutes, a change in the absorption maximum of the dye from 465 nm to 595 nm in acidic solutions ...
Main page; Contents; Current events; Random article; About Wikipedia; Contact us; Help; Learn to edit; Community portal; Recent changes; Upload file
Reference ranges (reference intervals) for blood tests are sets of values used by a health professional to interpret a set of medical test results from blood samples. Reference ranges for blood tests are studied within the field of clinical chemistry (also known as "clinical biochemistry", "chemical pathology" or "pure blood chemistry"), the ...
Main page; Contents; Current events; Random article; About Wikipedia; Contact us; Donate
The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is based on the reaction of Cu +, produced by the oxidation of peptide bonds, with Folin–Ciocalteu reagent (a mixture of phosphotungstic acid and phosphomolybdic acid in the Folin–Ciocalteu reaction).